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                      生物大分子國家重點實驗室

                      柳振峰  博士 研究員 博士生導師  

                      中科院生物物理所,生物大分子國家重點實驗室,研究組長

                      研究方向:膜蛋白結構生物學

                      電子郵件:liuzf@ibp.ac.cn

                      電       話:010-64881481 (office), 010-64889535 (lab)

                      通訊地址:北京市朝陽區大屯路15號(100101)

                      英文版個人網頁:http://english.ibp.cas.cn/faculty/index_18316.html?json=http://www.dengshan5.com/sourcedb_ibp_cas/cn/ibpexport/EN_xsszmL/202005/t20200519_5582991.json

                       

                      簡       歷:

                        1994 - 1998  廈門大學生物學系生物學專業,獲理學學士學位

                        1998 - 2004  中國科學院生物物理研究所,獲理學博士學位

                        2004 - 2010  加州理工學院化學與化工系/霍華德修斯醫學研究所,博士后/助理研究員

                        2011 - 至今  中國科學院生物物理研究所,研究員

                      獲獎及榮譽:

                       

                      社會任職:

                       

                      研究方向:

                        膜蛋白在細胞活動中承擔了各種各樣的重要生物學功能,例如光合作用中的光能吸收和轉化,呼吸作用中的電子傳遞和氧化磷酸化,物質的跨膜轉運,信號的跨膜轉導和膜內的酶促反應(如蛋白質降解,脂的合成和水解等)的催化等等。據估計,原核和真核細胞基因組中大約有20-30%的閱讀框編碼膜蛋白,凸顯膜蛋白研究的重要性。截止目前為止,蛋白質數據庫 (Protein Data Bank) 中僅僅有1%左右的結構數據是屬于膜蛋白的。膜蛋白的結構生物學研究大大落后于水溶性蛋白,主要是由于膜蛋白異源過量表達,純化和結晶過程中存在著眾多難以逾越的障礙。盡管如此,膜蛋白的結構生物學研究正在成為結構生物學領域的一個熱門的前沿分支,吸引了越來越多的研究者加入,目前在國際上正處于一個加速發展的前期(http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html)。

                        我實驗室的研究方向在于應用結構生物學為主的方法,如X-射線晶體學和冷凍電子顯微學,研究不同體系膜蛋白的結構與功能,如參與滲透壓調節的細菌機械敏感通道,磷脂生物合成和修飾的各種膜內酶,以及光合作用狀態轉換相關的膜蛋白復合物。目標是基于三維結構,對參與重要生物學過程的關鍵膜蛋白功能進行深入研究,闡明其發揮作用的分子機理。


                      菠菜光系統II-捕光復合物II超級復合物的三維結構(Wei X. P., Su X. D., et al. Nature 2016)

                      注:柳振峰課題組與章新政課題組和常文瑞課題組合作研究成果

                      三聚態胞內陽離子TRIC通道的結構與功能

                      (Yang H.T., Hu M.H., et al. Nature 2016)

                      脂類代謝相關的TMEM120A蛋白中含有一個輔酶A(CoASH)分子的結合位點

                      左:人源TMEM120A同質二聚體與CoASH分子形成的復合物

                      右:CoASH分子的結合誘導TMEM120A蛋白發生構象變化

                      (Rong, Y., Jiang J., Gao Y., et al. Elife 2021)

                      一種雙功能膜蛋白MprF介導的氨基酰磷脂合成和轉運機制及其與細菌耐受陽離子抗菌肽的關聯原理

                      (Song, D., Jiao H. & Liu Z. Nat. Commun. 2021)

                      承擔項目情況:

                       

                      代表論著:

                      1. Yang, B.W., Yao H.B., Li D.F. and Liu Z.F.*. The phosphatidylglycerol phosphate synthase PgsA utilizes a trifurcated amphipathic cavity for catalysis at the membrane-cytosol interface. Current Research in Structural Biology, in press (2021), DOI: https://doi.org/10.1016/j.crstbi.2021.11.005

                      2. Rong Y., Jiang J.H., Gao Y.W., Guo J.L., Song D.F., Liu W.H., Zhang M.M., Zhao Y.*, Xiao B.L.*, Liu Z.F.*. TMEM120A contains a specific coenzyme A-binding site and might not mediate poking- or stretch-induced channel activities in cells. Elife, 10, e71474 (2021).

                      3. Pan X.W., Tokutsu R., Li A.J., Takizawa K., Song C.H., Murata K., Yamasaki T., Liu Z.F.*, Minagawa J.* and Li M.*. Structural basis of LhcbM5-mediated state transitions in green algae. Nat. Plants,(2021) , DOI: https://doi.org/10.1038/s41477-021-00960-8

                      4. Sheng X., Liu Z.F.*, Kim E.,and Minagawa J.*. Plant and Algal PSII–LHCII Supercomplexes: Structure, Evolution and Energy Transfer. Plant and Cell Physiology, (2021) , DOI: https://doi.org/10.1093/pcp/pcab072

                      5. Song D.F., Jiao H.Z. and Liu Z.F.*. Phospholipid translocation captured in a bifunctional membrane protein MprF. Nat. Commun. 2:2927. (2021). (To read the article, please follow the Springer Nature SharedIt link at https://rdcu.be/ckO12)

                      6. Sheng X., Watanabe A., Li A.J., Kim E., Song C.H., Murata K., Song D.F., Minagawa J.* and Liu Z.F.*. Structural insight into light harvesting for photosystem II in green algae. Nat. Plants 5:1320-1330. (2019). (To read the article, please follow the Springer Nature SharedIt link at https://rdcu.be/bYFbR)

                      7. Jiao H.Z.,Yin Y and Liu Z.F.*. Structures of the Mitochondrial CDP-DAG Synthase Tam41 Suggest a Potential Lipid Substrate Pathway from Membrane to the Active Site. Structure 27, 1258–1269(2019).

                      8. Liu X. Y., Chai J.C., Ou X. M., Li M. and Liu Z.F.*. Structural Insights into Substrate Selectivity, Catalytic Mechanism, and Redox Regulation of Rice Photosystem II Core Phosphatase. Molecular Plant, 1-13, DOI: https://doi.org/10.1016/j.molp.2018.11.006. (2018).

                      9. Pan X.W., Ma J., Su X.D., Cao P., Chang W. R., Liu Z. F., Zhang X.Z.* and Li M.*. Structure of the maize photosystem I supercomplex with light-harvesting complexes I and II. Science, 360:1109-1113 (2018).

                      10. Sheng X., Liu X. Y., Cao P., Li M and Liu Z.F.*.  Structural roles of lipid molecules in the assembly of plant PSII-LHCII supercomplex. Biophys. Rep. 4, 189-203 (2018).

                      11. Li A.J. and Liu Z.F.*. Supramolecular structural basis of the light-harvesting process in plants. Prog. Biochem. Biophys.(生物化學與生物物理進展) 45, 935-946 (2018).

                      12. Cao P. ,Su X.D., Pan X.W., Liu Z. F., Chang W. R., and Li M.*.  Structure, assembly and energy transfer of plant photosystem II supercomplex. Biochim. Biophys. Acta Bioenergeticcs 1859, 633-644 (2018).

                      13. Su X.D., Ma J.,Wei X. P., Cao P., Zhu D.J., Chang W. R., Liu Z. F.*,  Zhang X.Z.* and Li M.*. Structure and assembly mechanism of plant C2S2M2-type PSII-LHCII supercomplex. Science, 357: 815-820 (2017).

                      14. Ou X. M., Guo J. L., Wang L. F., Yang H. T., Liu X. Y., Sun J. Y. and Liu Z. F.*. Ion- and water-binding sites inside an occluded hourglass pore of a trimeric intracellular cation (TRIC) channel. BMC Biology 15,31 (2017).

                      15. Yang H.T., Hu M.H., Guo J.L., Ou X.M., Cai T.X. and Liu Z. F.*. Pore architecture of TRIC channels and insights into their gating mechanism. Nature, 538, 537-541 (2016).

                      16. Wei X. P., Su X.D.,Cao P., Liu X. Y., Chang W. R., Li M.*, Zhang X.Z.* and Liu Z. F.*. Structure of spinach photosystem II- LHCII supercomplex at 3.2 Å resolution. Nature, 534, 69-74 (2016).

                      17. Li J., Guo J. L., Ou X. M., Zhang M. F., Li Y.Z. and Liu Z. F.* (2015). Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion. Proc. Natl. Acad. Sci. USA, 112: 10726-10731 (2015).

                      18. Wei X. P., Guo J. T., Li M.and Liu Z. F.* (2015). Structural Mechanism Underlying the Specific Recognition between the Arabidopsis State-Transition Phosphatase TAP38/PPH1 and Phosphorylated Light-Harvesting Complex Protein Lhcb1. Plant Cell, 27: 1113-1127. (2015, doi: 10.1105/tpc.15.00102).

                      19. Liu X. Y., Yan Y., Wu J. J. and Liu Z. F.* (2014). Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis. Nat. Commun. 5:4244. 

                      20. Pan X. W., Liu Z. F.*, Li M. & Chang W. R.*. (2013) Architecture and function of plant light-harvesting complexes II. Curr. Opin. Struct. Biol. 23:515-525. (* Corresponding authors) 

                      21. Guo J. T., Wei X. P., Li M., Pan X. W., Chang W. R.* & Liu Z. F.* (2013) Structure of the catalytic domain of a state transition kinase homolog from Micromonas algae. Protein & Cell 4, 607-619. (* Corresponding authors) 

                      22. Liu Z.F.,Walton T.A.,Rees D.C. (2010) A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators. Protein Science 19,808-814.

                      23. Liu Z.F.,Gandhi C.S.,Rees D.C. (2009) Structure of a tetrameric MscL in an expanded intermediate state. Nature 461,120-124.

                      24. Liu Z.F.,Chang W.R. (2008) Structure of the light-harvesting complex II. In: Photosynthetic Protein Complexes. Wiley-VCH (Book chapter) Fromme P. ed., 217-242.

                      25. Liu Z.F.,Chang W.R. (2008) Crystallization Methods of Membrane Proteins: Practical Aspects of Crystallizing Plant Light-Harvesting Complexes. In: Biophysical Techniques in Photosynthesis II. Spinger (Book chapter)Aartsma T. J. and Matysik J. eds. 26,77-96.

                      26. Yan H.C.,Zhang P.F.,Wang C.,Liu Z.F.,Chang W.R. (2007) Two lutein molecules in LHCII have different conformations and functions: Insights into the molecular mechanism of thermal dissipation in plants. Biochem. Biophys. Res. Commun. 355,457-463.

                      27. Pascal A.A.,Liu Z.F.,Broess K.,Oort B.V.,Amerongen H.V.,Wang C.,Horton P.,Robert B.,Chang W.R.,Ruban A. (2005) Molecular basis of photoprotection and control of photosynthetic light-harvesting. Nature 436,134-137.

                      28. Liu Z.F.,Yan H.C.,Wang K.B.,Kuang T.Y.,Zhang J.P.,Gui L.L.,An X.M.,Chang W.R. (2004) Crystal structure of spinach major light-harvesting complex at 2.72 Å resolution. Nature 428,287-292.

                       

                      (資料來源:柳振峰研究員,2022-02-16)

                       

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