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                      優秀青年科學基金獲得者

                      薛愿超  博士 研究員 博士生導師  

                      國家“杰出青年科學基金”獲得者
                      中科院生物物理所,中國科學院核酸生物學重點實驗室,研究組長

                      研究方向:(1)RNA結合蛋白與轉錄調控;(2)非編碼RNA功能基因組學

                      電子郵件:ycxue@ibp.ac.cn

                      電       話:010-64888447

                      通訊地址:北京市朝陽區大屯路15號(100101)

                      英文版個人網頁:http://english.ibp.cas.cn/faculty/index_18316.html?json=http://www.dengshan5.com/sourcedb_ibp_cas/cn/ibpexport/EN_xsszmX_Y/202005/t20200519_5582967.json

                       

                      簡       歷:

                        2000 - 2004  信陽師范學院,獲理學學士學位

                        2004 - 2010  武漢大學,獲理學博士學位

                        2010 - 2015  美國加州大學圣地亞哥分校,博士后

                        2015 - 至今  中國科學院生物物理研究所,研究員,博士生導師

                        2016 - 至今  中國科學院大學,崗位教授

                      獲獎及榮譽:

                       

                      社會任職:

                       

                      研究方向:

                        本研究組圍繞RNA結合蛋白和非編碼RNA,主要關注RNA-蛋白質復合物在轉錄和表觀遺傳層面如何調控基因表達,以及如何在細胞分化、轉分化和癌癥發生等命運決定過程中發揮作用。主要研究內容包括:

                        1、RNA結合蛋白的轉錄調控機制

                        RNA結合蛋白可在轉錄和轉錄后水平調控基因表達。除核酶外,絕大多數RNA需要與RNA結合蛋白形成復合物以發揮生物學功能,因此研究RNA與蛋白質的相互作用位點和模式是探索RNA功能的關鍵。我們關注RNA結合蛋白PTB,該家族共有三個成員:PTBP1(PTB)、PTBP2(nPTB)和PTBP3(ROD1)。它們都有4個與RNA結合的RRM結構域,氨基酸有~74%的相似度,但是功能卻不相同,而且表達的組織特異性也不一樣。我們將重點研究該蛋白家族成員在細胞分化和轉分化過程中的轉錄調控機制。

                        2、非編碼RNA的功能與作用機制研究

                        人類基因組的廣泛轉錄產生了海量的非編碼RNA,它們雖然也攜帶遺傳信息,但是往往不具備蛋白質編碼潛能,其調控功能主要通過形成復雜的高級結構并與其他RNA分子相互作用而實現。我們重點關注增強子RNA(enhancer RNA, eRNA)、啟動子RNA(promoter upstream antisense RNA, uaRNA)和長鏈非編碼RNA(long noncoding RNA),期望通過開發和利用多種RNA功能基因組學手段,系統研究這些非編碼RNA的生理功能與致病機理。

                        3、RNA-Protein研究的新技術開發

                        RNA在體內以高級結構形式存在,RNA結構的動態變化對于遺傳信息的精確傳遞至關重要,目前缺乏實驗方法在基因組范圍內解析RNA的原位高級結構。此外,研究RNA結合蛋白的經典CLIP-seq方法需要大量的細胞,無法解決細胞的異質性和個別細胞數目稀缺的問題,因此亟待開發單細胞的功能基因組學研究方法。

                      承擔項目情況:

                        

                      代表論著:

                      1. Changchang Cao#, Zhaokui Cai#, Xia Xiao#, Jian Rao, Juan Chen, Naijing Hu, Minnan Yang, Xiaorui Xing, Yongle Wang, Manman Li, Bing Zhou, Xiangxi Wang, Jianwei Wang*, Yuanchao Xue*. The architecture of the SARS-CoV-2 RNA genome inside virion. Nat Commun., 2021, 12(1): 3917.

                      2. Changchang Cao#, Zhaokui Cai#, Rong Ye, Ruibao Su, Naijing Hu, Hailian Zhao, Yuanchao Xue*. Global in situ profiling of RNA-RNA spatial interactions with RIC-seq. Nat Protoc., 2021, 16(6): 2916-2946.

                      3. Ruibao Su# , Li-Hua Fan#, Changchang Cao# , Lei Wang, Zongchang Du, Zhaokui Cai, Ying-Chun Ouyang, Yue Wang, Qian Zhou, Ligang Wu, Nan Zhang, Xiaoxiao Zhu, Wen-Long Lei, Hailian Zhao, Yong Tian, Shunmin He 1, Catherine C L Wong*, Qing-Yuan Sun*, Yuanchao Xue*. Global profiling of RNA-binding protein target sites by LACE-seq. Nat Cell Biol., 2021, 23(6): 664-675.

                      4. Rong Ye, Changchang Cao, Yuanchao Xue*. Enhancer RNA: biogenesis, function, and regulation. Essays Biochem, 2020, 64(6): 883-894.

                      5. Hao Qian, Xinjiang Kang, Jing Hu, Dongyang Zhang, Zhengyu Liang, Fan Meng, Xuan Zhang, Yuanchao Xue, Roy Maimon, Steven F Dowdy, Neal K Devaraj, Zhuan Zhou, William C Mobley, Don W Cleveland, Xiang-Dong Fu. Reversing a model of Parkinson's disease with in situ converted nigral neurons. Nature, 2020, 582(7813): 550-556.

                      6. Zhaokui Cai#; Changchang Cao#; Lei Ji#; Rong Ye; Di Wang; Cong Xia; Sui Wang; Zongchang Du; Naijing Hu; Xiaohua Yu; Juan Chen; Lei Wang; Xianguang Yang; Shunmin He; Yuanchao Xue*. RIC-seq for global in situ profiling of RNA-RNA spatial interactions, Nature, 2020, 582, 432-437

                      7. Juan Chen#, Zhaokui Cai#, Meizhu Bai#, Xiaohua Yu#, Chao Zhang#, Changchang Cao, Xihao Hu, Lei Wang, Ruibao Su, Di Wang, Lei Wang, Yingpeng Yao, Rong Ye, Baidong Hou, Yang Yu, Shuyang Yu, Jinsong Li, Yuanchao Xue*. The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes. Cell Research, 2018, 28: 981-995 (Cover story).

                      8. Yuanchao Xue#*, Hao Qian#, Jing Hu, Bing Zhou, Yu Zhou, Xihao Hu, Aziz Karakhanyan, Zhiping Pang, Xiang-Dong Fu*. Sequential Regulatory Loops as Key Gatekeepers for Neuronal Reprogramming in Human Cells. Nature Neuroscience 2016, 19(6): 807-15. doi: 10.1038/nn.4297.

                      9. Ashleigh E Schaffer, Veerle R C Eggens, Ahmet Okay Caglayan, Miriam S Reuter, Eric Scott, Nicole G Coufal, Jennifer L Silhavy, Yuanchao Xue, Hulya Kayserili , Katsuhito Yasuno, Rasim Ozgur Rosti, Mostafa Abdellateef, Caner Caglar, Paul R Kasher, J Leonie Cazemier, Marian A Weterman, Vincent Cantagrel, Na Cai, Christiane Zweier, Umut Altunoglu, N Bilge Satkin, Cengiz Yalcinkaya, Huseyin Caksen, Kaya Bilguvar, Xiang-Dong Fu, Christopher R Trotta, Stacey Gabriel, André Reis, Murat Gunel, Frank Baas, Joseph G Gleeson*. CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration. Cell, 2014, 157, 1-13.

                      10. Hong Ouyang#, Yuanchao Xue, Ying Lin, Xiaohui Zhang, Lei Xi, Sherrina Patel, Huimin Cai, Jing Luo, Meixia Zhang, Ming Zhang, Yang Yang, Gen Li, Hairi Li, Wei Jiang, Emily Yeh, Jonathan Lin, Michelle Pei, Jin Zhu, Guiqun Cao, Liangfang Zhang, Benjamin Yu, Shaochen Chen, Xiang-Dong Fu*, Yizhi Liu*, Kang Zhang*. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature, 2014, 511(7509): 358-361.

                      11. Yuanchao Xue#, Kunfu Ouyang, Jie Huang, Yu Zhou, Hong Ouyang, Hairi Li, Gang Wang, Qijia Wu, Chaoliang Wei, Yanzhen Bi, Li Jiang, Zhiqiang Cai, Hui Sun, Kang Zhang, Yi Zhang, Ju Chen, Xiang-Dong Fu*. Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated MicroRNA Circuits. Cell, 2013, 152 (1-2): 82-96.

                      12. Yuanchao Xue#, Yu Zhou#, Tongbin Wu, Tuo Zhu, Xiong Ji, Young-SooKwon, Chao Zhang, Gene Yeo, Douglas L. Black, Hui Sun, Xiang-Dong Fu*, Yi Zhang*, Genome-wide Analysis of PTB-RNA Interactions Reveals a Strategy Used by the General Splicing Repressor to Modulate Exon Inclusion or Skipping. Molecular Cell, 2009, 36(6): 996-1006 (Cover story).

                       

                      (資料來源:薛愿超研究員,2021-07-01)

                       

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